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The individual results for each type of cell line are as follow: a. HepG2 cells Within 24 hr there was a clear dose-dependent loss of cell proliferation compared to the vehicle-treated control (Fig. The effect became pronounced at doses higher than 1. With vehicle-treated control there were very few cell dead cells irrespective of the time in culture. There was a distinct threshold for cytotoxicity at doses higher than 11. The IC50 value for MSE cytotoxicity in this cell is estimated as 230.
Majno and Joris (1995) regarded necrosis as not the way of cell death but representative of the end stage manifestation of cell death. According to them upon receiving certain stimulus the cells may undergo apoptosis at low doses and necrosis at higher dose and sometimes both apoptotic and necrotic features present in the same cells. At the end of apoptotic death if the cells fail to be engulfed by neighbour cells or macrophages then cells may die by necrosis as the plasma membrane and cellular energy were compromised.
According to them upon receiving certain stimulus the cells may undergo apoptosis at low doses and necrosis at higher dose and sometimes both Bali Kratom Vs Opiates Stamford apoptotic and necrotic features present in the same cells. At the end of apoptotic death if the cells fail to be engulfed by neighbour cells or macrophages then cells may die by necrosis as the plasma membrane and cellular energy were compromised. Majno and Joris 1995). Various in vitro test systems are available to determine the cell death upon xenobiotic insult.
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Increases in p53 levels can also lead to increased kratom withdrawal high blood pressure expression of numerous p53 target genes and one of the most important is cyclin-dependant kinase inhibitor A (CDKN1A) or p21. Cdk inhibitor p21 (p21CIP1) is also regarded as a downstream effector gene (Pellegata et al 1996). Human p21 gene located at chromosome 6 can act as a regulator for cell cycle progression controlled by p53 (Gartel and Radakrishnan 2005). Thus the positive kratom extract dosage 80x lovelock links between p53 and its effector gene p21 lead to binding of p21 to Cyclin-Cdks complexes which in turn inhibit the cells in G1 phase (Morgan 2007). Structural organisation of p53 protein.
Serum free media was added to respective wells and treated with various concentrations of MSE. Triplicate wells of 10% FBS media for control group were also added for comparison. After 24 hr incubation the medium was aspirated
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and the cells were washed with PBS.