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SH-SY5Y cells (105 cells per well) were seeded in 6 well plates and treated with various concentrations of MSE and MIT for the designated time period. Cells were harvested by routine trypsinisation procedure as described in chapter 2 (section 2. After the centrifugation process the supernatant was aspirated and the cell pellet was washed with PBS followed by centrifugation (1000 r.

By 48 hr proliferation of cells treated with the lowest concentration of MSE (1. As with the HepG2 cells MSE associated cell death was only apparent at doses higher than 11. The IC50 for this cell at 24 hr period is 410.

There was no significant difference in cell numbers compared to negative control or positive control groups; however based on the formula which takes into account the suspension growth for two days culturing period low dose-dependant RSG was calculated. The low suspension growth was noted even after 24 hr post bali kratom effects treatment (data not shown). Thus all concentration tested in

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this group were chosen for plating for the final step of assessment.

To further clarify the above finding S9 from rat liver premium red vein indo kratom (induced by Arochlor 1254) was used with SH-SY5Y and HEK-293 cells as

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these cells have no metabolic activity. MSE in SHSY5Y and HEK 293 cells respectively; this cytotoxic dose of MSE is ten fold lower than with cells treated without S9. CYP 1A2 inhibitor) and Is Kratom kratom tincture canada bulls gap Legal In Ecuador Harrisburg 3-amino 124triazole (CYP 2E1 inhibitor) were used with MCL-5 cells and analysed for cytotoxicity.